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KMID : 1134820170460070857
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 7 p.857 ~ p.867
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Preparation and Characterization of Double-Layered Coated Capsule Containing Low Molecular Marine Collagen and γ-Aminobutyric Acid Producing Lactobacillus brevis CFM20
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Kim Sun-Yeong
Oh Do-Geon
Kim Kwang-Yup
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Abstract
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This study was performed to encapsulate low molecular weight marine collagen and γ-aminobutyric acid (GABA)-producing lactic acid bacteria to inhibit degradation and improve survival rate during exposure to adverse conditions of the gastro-intestinal tract. Calcium-alginate method was used for the manufacture of a double-layered coated capsule. The inner core material was composed of collagen and lactic acid bacteria, and the coating materials were alginate and chitosan. The sizes and shapes of the double-coated capsule were affected mainly by centrifuge speed and pH. Manufactured capsules were observed with a scanning electron microscope and by confocal laser scanning microscopy to confirm the micromorphological changes of capsules and bacterial cells. As a result, double-layered coated capsules were not degraded at pH 1.2, whereas degradation occurred at pH 7.4. In addition, GABA and collagen were maintained in stable state at pH 1.2. Therefore, double-layered coated capsules developed in this study would not be degraded in the stomach and could be stably delivered to the small intestine to benefit intestinal and dermatic health.
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KEYWORD
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lactic acid bacteria, γ-aminobutyric acid (GABA), low molecule marine collagen, calcium-alginate capsulation
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